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Image Search Results
Journal: Anesthesiology
Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model
doi: 10.1097/aln.0b013e31823de68d
Figure Lengend Snippet: Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti
Techniques: Reverse Transcription, Polymer, Expressing, Control
Journal: Anesthesiology
Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model
doi: 10.1097/aln.0b013e31823de68d
Figure Lengend Snippet: Fig. 3. Changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 protein expression over time in tumor- bearing mice and sham group mice. Western blot for -actin, GFAP, mGluR3, and mGluR5 resulted in products of 45, 50, 110 and 150 kDa, as expected (markers show predicted band sizes) (A and B). Densitometric quantification of -actin, GFAP, mGluR3, and mGluR5 immunoreactivity on Western blots (C, D, and E). Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti
Techniques: Expressing, Western Blot, Control
Journal: Anesthesiology
Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model
doi: 10.1097/aln.0b013e31823de68d
Figure Lengend Snippet: Fig. 5. Effects of chronic intrathecal administration of APDC (mGluR3 agonist,150 nmol/5 l), LY 341495 (mGluR3 antag- onist,15 nmol/5 l), CHPG (mGluR5 agonist, 300 mol/5 l), MTEP (mGluR5 antagonist, 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on hind limb pain behaviors. (A) The num- ber of flinches during 2 min. (B) Paw withdrawal mechanical threshold to von Frey filaments. (C) Paw withdrawal thermal latencyto radiant heat. Animals were treated once daily on days 14–20 after surgery with the drugs or vehicles. Pain behaviors were assessed on day 7 after the first intrathecal injection. Ipsi ipsilateral hind limb to the surgery; Contra Contralateral hind limb to the surgery. Each column repre- sented mean SD for eight animals. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).
Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti
Techniques: Saline, Injection
Journal: Anesthesiology
Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model
doi: 10.1097/aln.0b013e31823de68d
Figure Lengend Snippet: Fig. 6. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on spinal glial fibrillary acidic protein (GFAP) messenger RNA (mRNA) (A) and protein (B and C) expression. Animals were treated with once-daily injections on days 14–20 after surgery with the drugs or vehicles. The spinal cord was removed on day 7 after the first intrathecal injection. Each group used five mice. The mRNA and protein data were presented as fold change of control (day 0) mean SD. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).
Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti
Techniques: Saline, Expressing, Injection, Control
Journal: Anesthesiology
Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model
doi: 10.1097/aln.0b013e31823de68d
Figure Lengend Snippet: Fig. 7. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on tumor growth. The ratio of maximum thigh circumference (ipsi/contra) of mice indicated the size of the tumor. Animals were treated once daily injections on days 14–20 after sur- gery with the drugs or vehicles. Data were presented as mean SD. If the two groups were marked with different letters (a, b), it showed the data were of statistical difference between these two groups (P 0.05).
Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti
Techniques: Saline