goat polyclonal anti collagen type 3 Search Results


95
SouthernBiotech goat anti type 3 collagen antibody
Goat Anti Type 3 Collagen Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech biotinylated goat anti rabbit igg
Biotinylated Goat Anti Rabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SouthernBiotech goat anti collagen type 3
Goat Anti Collagen Type 3, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
SouthernBiotech anti type 3
Anti Type 3, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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91
Santa Cruz Biotechnology mglur3
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Mglur3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech anti type 3 collagen
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Anti Type 3 Collagen, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad anti tag secondary antibodies
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Anti Tag Secondary Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abcam marker
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Marker, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Bio-Rad mouse monoclonal anti type 3 complement receptor igg2 a
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Mouse Monoclonal Anti Type 3 Complement Receptor Igg2 A, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson anti–type 3 insp3r mab
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Anti–Type 3 Insp3r Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Rockland Immunochemicals rabbit anti collagen type 3
Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), <t>mGluR3,</t> and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.
Rabbit Anti Collagen Type 3, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.

Journal: Anesthesiology

Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model

doi: 10.1097/aln.0b013e31823de68d

Figure Lengend Snippet: Fig. 2. Quantitative real-time reverse transcription-polymer- ase chain reaction analysis of changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 messenger RNA (mRNA) expression after the surgery on mice. Spinal GFAP (A), mGluR3 (B), and mGluR5 (C) mRNA expression progres- sively increased as time went on after day 7 in tumor-bearing mice. Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.

Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti mGluR3 (goat affinity purified polyclonal antibody; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), anti mGluR5 (rabbit affinity purified polyclonal antibody; 1:500; Abcam, Hong Kong, China), anti-GFAP (goat affinity purified polyclonal antibody; 1:1000; Santa Cruz Biotechnology) or anti- -actin (rabbit affinity purified polyclonal antibody; 1:1000;Cell Signaling, Danvers, MA) diluted in blocking buffer.

Techniques: Reverse Transcription, Polymer, Expressing, Control

Fig. 3. Changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 protein expression over time in tumor- bearing mice and sham group mice. Western blot for -actin, GFAP, mGluR3, and mGluR5 resulted in products of 45, 50, 110 and 150 kDa, as expected (markers show predicted band sizes) (A and B). Densitometric quantification of -actin, GFAP, mGluR3, and mGluR5 immunoreactivity on Western blots (C, D, and E). Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.

Journal: Anesthesiology

Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model

doi: 10.1097/aln.0b013e31823de68d

Figure Lengend Snippet: Fig. 3. Changes of spinal glial fibrillary acidic protein (GFAP), mGluR3, and mGluR5 protein expression over time in tumor- bearing mice and sham group mice. Western blot for -actin, GFAP, mGluR3, and mGluR5 resulted in products of 45, 50, 110 and 150 kDa, as expected (markers show predicted band sizes) (A and B). Densitometric quantification of -actin, GFAP, mGluR3, and mGluR5 immunoreactivity on Western blots (C, D, and E). Each group used five mice. Data were presented as fold change of control (day 0) mean SD. # P 0.05 versus day 0. * P 0.05 versus sham mice.

Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti mGluR3 (goat affinity purified polyclonal antibody; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), anti mGluR5 (rabbit affinity purified polyclonal antibody; 1:500; Abcam, Hong Kong, China), anti-GFAP (goat affinity purified polyclonal antibody; 1:1000; Santa Cruz Biotechnology) or anti- -actin (rabbit affinity purified polyclonal antibody; 1:1000;Cell Signaling, Danvers, MA) diluted in blocking buffer.

Techniques: Expressing, Western Blot, Control

Fig. 5. Effects of chronic intrathecal administration of APDC (mGluR3 agonist,150 nmol/5 l), LY 341495 (mGluR3 antag- onist,15 nmol/5 l), CHPG (mGluR5 agonist, 300 mol/5 l), MTEP (mGluR5 antagonist, 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on hind limb pain behaviors. (A) The num- ber of flinches during 2 min. (B) Paw withdrawal mechanical threshold to von Frey filaments. (C) Paw withdrawal thermal latencyto radiant heat. Animals were treated once daily on days 14–20 after surgery with the drugs or vehicles. Pain behaviors were assessed on day 7 after the first intrathecal injection. Ipsi ipsilateral hind limb to the surgery; Contra Contralateral hind limb to the surgery. Each column repre- sented mean SD for eight animals. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).

Journal: Anesthesiology

Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model

doi: 10.1097/aln.0b013e31823de68d

Figure Lengend Snippet: Fig. 5. Effects of chronic intrathecal administration of APDC (mGluR3 agonist,150 nmol/5 l), LY 341495 (mGluR3 antag- onist,15 nmol/5 l), CHPG (mGluR5 agonist, 300 mol/5 l), MTEP (mGluR5 antagonist, 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on hind limb pain behaviors. (A) The num- ber of flinches during 2 min. (B) Paw withdrawal mechanical threshold to von Frey filaments. (C) Paw withdrawal thermal latencyto radiant heat. Animals were treated once daily on days 14–20 after surgery with the drugs or vehicles. Pain behaviors were assessed on day 7 after the first intrathecal injection. Ipsi ipsilateral hind limb to the surgery; Contra Contralateral hind limb to the surgery. Each column repre- sented mean SD for eight animals. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).

Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti mGluR3 (goat affinity purified polyclonal antibody; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), anti mGluR5 (rabbit affinity purified polyclonal antibody; 1:500; Abcam, Hong Kong, China), anti-GFAP (goat affinity purified polyclonal antibody; 1:1000; Santa Cruz Biotechnology) or anti- -actin (rabbit affinity purified polyclonal antibody; 1:1000;Cell Signaling, Danvers, MA) diluted in blocking buffer.

Techniques: Saline, Injection

Fig. 6. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on spinal glial fibrillary acidic protein (GFAP) messenger RNA (mRNA) (A) and protein (B and C) expression. Animals were treated with once-daily injections on days 14–20 after surgery with the drugs or vehicles. The spinal cord was removed on day 7 after the first intrathecal injection. Each group used five mice. The mRNA and protein data were presented as fold change of control (day 0) mean SD. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).

Journal: Anesthesiology

Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model

doi: 10.1097/aln.0b013e31823de68d

Figure Lengend Snippet: Fig. 6. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on spinal glial fibrillary acidic protein (GFAP) messenger RNA (mRNA) (A) and protein (B and C) expression. Animals were treated with once-daily injections on days 14–20 after surgery with the drugs or vehicles. The spinal cord was removed on day 7 after the first intrathecal injection. Each group used five mice. The mRNA and protein data were presented as fold change of control (day 0) mean SD. If the two groups were marked with different letters (a, b, c, or d), it showed the data were of statistical difference between these two groups (P 0.05).

Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti mGluR3 (goat affinity purified polyclonal antibody; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), anti mGluR5 (rabbit affinity purified polyclonal antibody; 1:500; Abcam, Hong Kong, China), anti-GFAP (goat affinity purified polyclonal antibody; 1:1000; Santa Cruz Biotechnology) or anti- -actin (rabbit affinity purified polyclonal antibody; 1:1000;Cell Signaling, Danvers, MA) diluted in blocking buffer.

Techniques: Saline, Expressing, Injection, Control

Fig. 7. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on tumor growth. The ratio of maximum thigh circumference (ipsi/contra) of mice indicated the size of the tumor. Animals were treated once daily injections on days 14–20 after sur- gery with the drugs or vehicles. Data were presented as mean SD. If the two groups were marked with different letters (a, b), it showed the data were of statistical difference between these two groups (P 0.05).

Journal: Anesthesiology

Article Title: Intrathecal Injection of Metabotropic Glutamate Receptor Subtype 3 and 5 Agonist/Antagonist Attenuates Bone Cancer Pain by Inhibition of Spinal Astrocyte Activation in a Mouse Model

doi: 10.1097/aln.0b013e31823de68d

Figure Lengend Snippet: Fig. 7. Effects of intrathecal administration of APDC (mGluR3 agonist; 150 nmol/5 l), LY 341495 (mGluR3 antagonist; 15 nmol/5 l), CHPG (mGluR5 agonist; 300 mol/5 l), MTEP (mGluR5 antagonist; 150 nmol/5 l), morphine (5 mol/5 l), fluorocitrate (0.75 nmol/5 l), Vehicle 1 (dilute NaOH in 0.9% saline; 5 l) and Vehicle 2 (dilute DMSO in 0.9% saline; 5 l) on tumor growth. The ratio of maximum thigh circumference (ipsi/contra) of mice indicated the size of the tumor. Animals were treated once daily injections on days 14–20 after sur- gery with the drugs or vehicles. Data were presented as mean SD. If the two groups were marked with different letters (a, b), it showed the data were of statistical difference between these two groups (P 0.05).

Article Snippet: Membranes were blocked in PBS/5% skim milk/0.1% Tween 20 for 2 h at room temperature, followed by overnight incubation at 4°C with anti mGluR3 (goat affinity purified polyclonal antibody; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), anti mGluR5 (rabbit affinity purified polyclonal antibody; 1:500; Abcam, Hong Kong, China), anti-GFAP (goat affinity purified polyclonal antibody; 1:1000; Santa Cruz Biotechnology) or anti- -actin (rabbit affinity purified polyclonal antibody; 1:1000;Cell Signaling, Danvers, MA) diluted in blocking buffer.

Techniques: Saline